human timp 2 duoset elisa  (R&D Systems)

Journal: iScience

Article Title: Human monocyte-derived macrophages shift subcellular metalloprotease activity depending on their activation state

doi: 10.1016/j.isci.2024.111171

Figure Lengend Snippet:

Article Snippet: MMP-12 (Human MMP-12 ELISA, Cat # EH327RB, Invitrogen), ADAM-9 (Human ADAM-9 DuoSet ELISA, Cat # DY939, R&D Systems), TIMP-1 (Human TIMP-1 DuoSet ELISA, Cat # DY970, R&D Systems), TIMP-2 (Human TIMP-2 DuoSet ELISA, Cat # DY971, R&D Systems), MMP-9/TIMP-1 complex (Human MMP-9/TIMP-1 DuoSet ELISA, Cat # DY1449, R&D Systems), Cystatin C (Human Cystatin C DuoSet ELISA, Cat # DY1196, R&D Systems), Cathepsin D (Human Cathepsin D DuoSet ELISA, Cat # DY1014, R&D Systems), Serpin E1 (Human Serpin E1 DuoSet ELISA, Cat # DY1786, R&D Systems), Tumor Necrosis Factor alpha (TNF-α) (Human TNF-alpha DuoSet ELISA, Cat # DY210, R&D Systems), IL-1β (Human IL-1 beta DuoSet ELISA, Cat # DY201, R&D Systems), IL-6 (Human IL-6 DuoSet ELISA, Cat # DY206, R&D Systems) and CCL-18 (Human CCL18/PARC DuoSet ELISA, Cat # DY394, R&D Systems) concentrations were measured by sandwich ELISA according to the manufacturer’s protocols.

Techniques: Plasmid Preparation, Recombinant, Knock-Out, Blocking Assay, Staining, Antibody Labeling, Reverse Transcription, Lysis, Protease Inhibitor, Enzyme-linked Immunosorbent Assay, Fractionation, Cell Culture, Software, Flow Cytometry, Fluorescence, Spectrophotometry

Journal: Current Issues in Molecular Biology

Article Title: Taurine and Polyphenol Complex Repaired Epidermal Keratinocyte Wounds by Regulating IL8 and TIMP2 Expression

doi: 10.3390/cimb46080512

Figure Lengend Snippet: Inflammatory cytokine/chemokine profiles induced by C. acnes subtypes.

Article Snippet: The expression levels of IL8 and TIMP2 were evaluated via ELISA, using the Human IL8/CXCL8 DuoSet ELISA Kit (DY208) and Human TIMP2 DuoSet ELISA Kit (DY971, R&D systems, Minneapolis, MN, USA), as per the manufacturer’s protocol.

Techniques: Control

Journal: Current Issues in Molecular Biology

Article Title: Taurine and Polyphenol Complex Repaired Epidermal Keratinocyte Wounds by Regulating IL8 and TIMP2 Expression

doi: 10.3390/cimb46080512

Figure Lengend Snippet: Comparative analysis of IL8 and TIMP2 expression in normal skin, acne-affected, inflamed, and pigmented sites. IL8 and TIMP2 protein expression were quantified from (1) non-acneic skin, (2) normal skin, (3) sites of inflammation, and (4) sites of pigmentation in acneic skin by ELISA. * p < 0.05 versus control group; Student’s t -test. Data are expressed as the mean ± SEM.

Article Snippet: The expression levels of IL8 and TIMP2 were evaluated via ELISA, using the Human IL8/CXCL8 DuoSet ELISA Kit (DY208) and Human TIMP2 DuoSet ELISA Kit (DY971, R&D systems, Minneapolis, MN, USA), as per the manufacturer’s protocol.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Control

Journal: Current Issues in Molecular Biology

Article Title: Taurine and Polyphenol Complex Repaired Epidermal Keratinocyte Wounds by Regulating IL8 and TIMP2 Expression

doi: 10.3390/cimb46080512

Figure Lengend Snippet: IL8 and TIMP2 regulatory effects of chlorogenic acid and taurine in cultured HaCaT cells. The synergistic effect of chlorogenic acid and taurine on ( A ) IL8 inhibition, and ( B ) TIMP2 activation after 24 h of co-treatment with heat-killed C. acnes . * p < 0.05, ** p < 0.01, *** p < 0.001 relative to the control group; Student’s t -test. Data are expressed as mean ± SEM.

Article Snippet: The expression levels of IL8 and TIMP2 were evaluated via ELISA, using the Human IL8/CXCL8 DuoSet ELISA Kit (DY208) and Human TIMP2 DuoSet ELISA Kit (DY971, R&D systems, Minneapolis, MN, USA), as per the manufacturer’s protocol.

Techniques: Cell Culture, Inhibition, Activation Assay, Control